Effect of Papaya flower ethanolic extract (Carica Papaya) on the time kill of tetracyclin against Escherichia Coli

Papaya ( Carica papaya ) is found throughout Indonesia. flowers contain saponin, tannin, alkaloid, and flavonoid compounds which functions as antibacterial. Escherichia coli a Gram-negative rod bacteria that could be in the human large intestine cause of diarrhea. The study aimed to determine effect flower extract combination with tetracycline antibiotics using smallest concentration capable inhibiting 50% bacterial growth against ATCC 25922 time-kill test method. This was method Minimum Inhibitory Concentration (MIC) . MIC ethanol 2 mg/mL. showed death phase occurred at 4 - 24 hours. bacteriostatic obtained 0 hours hours, while antibiotic its extracts had bactericidal 8